Association of polymorphisms of genes SLC30A8 and MC4R with the prognosis of the development of type 2 diabetes mellitus

BACKGROUND : The prevalence of Type 2 diabetes mellitus (T2DM) has reached epidemic proportions and it is estimated to affect over 400 million people worldwide. Moreover, the incidence expected continue rise projected nearly one three individuals by year 2050. These alarming projections suggest that there an urgent need for development implementation novel prevention treatment strategies combat in T2DM. AIM To study possibility using polymorphisms genes SLC30A8 MC4R as markers predicting T2D population Novosibirsk. MATERIALS AND METHODS: On basis prospective follow-up a representative sample residents Novosibirsk (The HAPIEE Project), groups were formed according “case-control” principle (case — who had 10 years follow-up, control did not developed disorders carbohydrate metabolism). group (n = 443, mean age 56.2 ± 6.7 years, men 29.6%, women 70.4%), 532, 56.1 7.1 32.7%, 67.3%). DNA was isolated phenol-chloroform extraction. Genotyping performed method polymerase chain reaction with subsequent analysis restriction fragment length polymorphism, real time. Statistical processing carried out SPSS 16.0 software package. RESULTS Genotype TT rs13266634 gene associated risk developing (relative RR 1.51, 95% confidence interval CI 1.11–2.05, p =0.008). CC genotype protective effect against (RR 0.57, 0.35–0.92, p=0.026). No significant rs17782313 on found. CONCLUSION polymorphism confirmed its association prognosis T2D, which indicates considering candidate inclusion score. between